Composite

Part:BBa_K2448058:Design

Designed by: Jeremy Armetta   Group: iGEM17_Evry_Paris-Saclay   (2018-12-27)


Psicose Biosensor pPsiA-At PsiR-At (mEmerald) with D-Psicose 3-epimerase from Clostridium cellulolyt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1337
    Illegal XhoI site found at 62
    Illegal XhoI site found at 2645
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 791
    Illegal AgeI site found at 1819
    Illegal AgeI site found at 1942
    Illegal AgeI site found at 3279
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biosensor was built using the Psicose Biosensor pPsiA-PsiR from Agrobacterium tumefaciens to which we added downstream the D-Psicose-3-Epimerase from C. cellulolyticum (BBa_K2448021).


Source

DNA synthesis & assembly

References